We performed scRNAseq from corpus callosum (CC) tissue of hES-derived Shiverer chimeras at 10 weeks of age. Viable whole cells (human and mouse mixed) were collected and barcoded cDNA libraries were generated for each timepoint using the Chromium Next GEM Single Cell 3' GEM, Library and Gel Bead Kit v3.1 (10X Genomics®), according to manufacturer’s instructions. Cells from different species were bioinformatically separated and separated into two distinct datasets. Dataset 1 contained samples of hES-derived chimeric oligodendroglia treated with either metformin of vehicle (ddH2O) for three weeks, while Dataset 2 contained samples of hES-derived chimeric oligodendroglia treated with either clemastine of vehicle (DMSo) for three weeks Here, I have uploaded the DE analysis output for each dataset, containing all markers or filtered markers (p ≤ 0.05, fold-change expressed logarithmically (logFC) ≥ 0.5) of each sub-cluster. I have also uploaded the DE analysis outpute for each dataset comparing treated and untreated cells of all clusters. Upregulated markers in the control groups are represented by positive values, while upregulated markers in the treated groups are represented by negative values.