seqfile = lwsa_FW_k1o05_1030.phy ** sequence data filename treefile = bel_38_FW.phy ** tree structure file name outfile = lwsa_FW_k1o05_1030_OUTPUT ** main result file name noisy = 9 * 0,1,2,3,9: how much rubbish on the screen verbose = 0 * 0: concise; 1: detailed, 2: too much runmode = 0 * 0: user tree; 1: semi-automatic; 2: automatic * 3: StepwiseAddition; (4,5):PerturbationNNI; -2: pairwise seqtype = 1 * 1:codons; 2:AAs; 3:codons-->AAs CodonFreq = 2 ** 0:1/61 each, 1:F1X4, 2:F3X4, 3:codon table ** 4:F1x4MG, 5:F3x4MG, 6:FMutSel0, 7:FMutSel * estFreq = 0 *0: frequency/fitness parameters calculated from observed frequencies in data *1: frequency/fitness parameters estimated by ML from the data (use with FMutSel models) * ndata = 10 clock = 0 * 0:no clock, 1:clock; 2:local clock; 3:CombinedAnalysis aaDist = 0 * 0:equal, +:geometric; -:linear, 1-6:G1974,Miyata,c,p,v,a aaRatefile = dat/jones.dat * only used for aa seqs with model=empirical(_F) * dayhoff.dat, jones.dat, wag.dat, mtmam.dat, or your own model = 0 ** models for codons: * 0:one, 1:b free ratio, 2:2 or more dN/dS ratios for branches 3:Clade model C * models for AAs or codon-translated AAs: * 0:poisson, 1:proportional, 2:Empirical, 3:Empirical+F * 6:FromCodon, 7:AAClasses, 8:REVaa_0, 9:REVaa(nr=189) NSsites = 0 1 2 3 **0:one w (M0); 1:neutral (M1a); 2:selection (M2a); 3:discrete (M3) ;4:freqs; * 5:gamma; 6:2gamma; 7:beta (M7); 8:beta&w (M8); 9:betaγ * 10:beta&gamma+1; 11:beta&normal>1; 12:0&2normal>1; * 13:3normal>0 * 22:M2a_Rel icode = 0 * 0:universal code; 1:mammalian mt; 2-10:see below Mgene = 0 * codon: 0:rates, 1:separate; 2:diff pi, 3:diff kapa, 4:all diff * AA: 0:rates, 1:separate fix_kappa = 0 ** 1: kappa fixed, 0: kappa to be estimated kappa = 1 ** initial or fixed kappa fix_omega = 0 ** 1: omega or omega_1 fixed, 0: estimate **fix omega at one in M8 for M8a omega = 0.5 ** initial or fixed omega, for codons or codon-based AAs fix_alpha = 1 * 0: estimate gamma shape parameter; 1: fix it at alpha alpha = 0 * initial or fixed alpha, 0:infinity (constant rate) Malpha = 0 * different alphas for genes ncatG = 3 * # of categories in dG of NSsites models getSE = 0 * 0: don't want them, 1: want S.E.s of estimates RateAncestor = 0 * (0,1,2): rates (alpha>0) or ancestral states (1 or 2) Small_Diff = .5e-6 cleandata = 0 * remove sites with ambiguity data (1:yes, 0:no)? * fix_blength = -1 * 0: ignore, -1: random, 1: initial, 2: fixed method = 1 * Optimization method 0: simultaneous; 1: one branch a time * Genetic codes: 0:universal, 1:mammalian mt., 2:yeast mt., 3:mold mt., * 4: invertebrate mt., 5: ciliate nuclear, 6: echinoderm mt., * 7: euplotid mt., 8: alternative yeast nu. 9: ascidian mt., * 10: blepharisma nu. * These codes correspond to transl_table 1 to 11 of GENEBANK.